determining iron content in foods by spectrophotometry

2001). A comparative study of the determination of iron in a wide variety of foods was carried out using the atomic absorption spectrophotometric (AAS) and phenanthroline colorimetric methods. Reusser, 1983. The iron content in the complexed form decreased, while the content of Fe(II) increased. 1). This suggests that, regardless of the total iron content in extracts, the element is found in the forms whose participation in the total iron content is similar.
Nat Biotechnol 17:282–286, Harvey AE, Smart JA, Amis ES (1955) Simultaneous spectrophotometric determination of iron (II) and total iron with 1,10-phenanthroline. Sylwia Zalicka declares that she has no conflict of interest. Standard solutions of iron species Fe(II) and Fe(III) of concentration 1.00 mg ml−1 were prepared from weights of the following salts: ferrous ammonium sulphate hexahydrate and ferric ammonium sulphate dodecahydrate (both chemicals obtained from Acros). Niedzielski, P., Zielinska-Dawidziak, M., Kozak, L. et al. The iron speciation during the extraction in room temperature was confirmed, as well as its disturbance when the samples were heated. Sequential injection spectrophotometric determination of iron as Fe (II) in multivitamin preparations using 1,10-phenanthroline as complexing agent. Flour fortification with iron is an attractive optimum for controlling iron-deficiency anemia in countries such as Iran. Iron working standard solution were prepared by diluting be obtained (Rao, 2005). Seyed Jalal Hosseinimehr, iron is present in fortified flour, which is critical to success of flour fortification PubMed Google Scholar. equation was y = 0.0765x+0.0057 with a correlation coefficient (R2) J Nutr 133:1490–1493, Lozano-Camargo ML, Rojas-Hernandez A, Gomez-Hernandez M, Pacheco-Hernandez ML, Galicia L, Ramirez-Silva MT (2007) UV–visible spectroscopic and electrochemical study of the complex formation between Fe(II) and 5-amino-1,10-phenantroline (5-Aphen) in aqueous solution. 501 0 obj <>/Filter/FlateDecode/ID[<2EED7E44714B5647808996EDD9D51DBF><2CB643B2B17C294EA233DF97A5676B75>]/Index[479 56]/Info 478 0 R/Length 111/Prev 154334/Root 480 0 R/Size 535/Type/XRef/W[1 3 1]>>stream Chem Anal (Warsaw) 42:411–416, Kozak L, Niedzielski P, Wachowiak W (2013) The tandem analytical method of flow injection diode array spectrophotometry and flame atomic absorption spectrometry (FI DAD(Vis)-FAAS) in iron speciation studies using 1,10-phenanthroline complexes. Talanta, 37: 711-718.Tesfaldet, Z.O. is inexpensive and safely compared to atomic absorption. There are two types of nutritional iron usually distinguished: heme (stable porphyrin complexes) and non-heme (from weak complexes or ‘free’ iron). and M.E.

grade Fe wire in 20 mL HCl and water and dilute to 1 L. Dilute 100 mL of this This study showed that spectrophotometric method The total content of iron was determined using atomic absorption spectrometry with air-acetylene flame atomization. Measure absorption of solution directly with atomic absorption spectrophotometer.
It may, therefore, be assumed that this is the only extraction procedure that leads to obtaining representative results of total iron content in a sample. developed country. 2 = 0.9912), detection limit 1 mg kg−1, precision 1.4 % for 10 mg kg−1 in Fe(II) determinations, slope of calibration curve 0.1308 (mg l−1)−1, correlation coefficient (R The atomic absorption The paper presents the determination of iron forms in food products. volume 7, pages2023–2032(2014)Cite this article. Google Scholar, Hosseinimehr SJ, Bagheri G, Gholipoor M, Mokarami H (2001) Comparative spectrophotometric and atomic absorption determination of iron content in wheat flour. The procedure applied in the determinations of iron forms in geochemical studies of sediments and soils could not be used in the studies of food due to the colour of extracts and potential presence of sample components in the form of colloid solutions, hence the necessity to develop a new methodology which would enable the analyses of food products. (spectrophotometric method) is practical, perfect and not very skilled personal

For instance, the method based on iron reaction with 1,10-phenanthroline has been used in selective determinations of Fe(II) and Fe(III) contents. Lidia Kozak declares that she has no conflict of interest. Then, 2.0 g of a sample should be extracted with 20.0 ml 2.0 mol l−1 hydrochloric acid, simultaneously at room temperature (approx. 30 %.

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